Flag Flagm2 6His histidine his-tag gst gsh glutathione streptavidin anti-human anti-mouse anti-rabbit homogeneous alphascreen alphalisa alphaplex conjugation conjugated LOCI biotin digoxygenin dig protac protein protein-protein TR-FRET lance thunder htrf immunoassay immuno-oncology immuno oncology cytokines IL-1b Interleukin IL-2 IL-6 IL-8 INF-gamma interferon TNF-alpha TNF biomarker
ENLIGHT OMEGA
(Oxygen Mediated/Gated Assay)
ENLIGHT OMEGA
(Oxygen Mediated/Gated Assay)
TM
TM
ENLIGHT OMEGA (Oxygen MEdiated/Gated Assay) is a novel non-wash (homogeneous) assay technology using a unique combination of Donor (Sensibeads) and Acceptor (Chemibeads) nanoparticles each embedded with proprietary chemicals (patent pending). ENLIGHT OMEGA assays allows one to measure the interaction occuring between two binding partners (A and B) respectively captured onto Sensibeads and Chemibeads. Singlet oxygen, produced upon excitation of the Sensibeads at 680nm, travels up to 200nm in aqueous solution and reacts with specific compounds embedded in the Chemibeads when located in close proximity. The latter beads then emit a signal detectable at 615nm which is proportional to the level of interaction prevailing between the binding partners A and B.
Contrasting with Alpha Donor beads, our Sensibeads 680 are not light sensitive to ambien light!!!
Due to their 200nm proximity allowance, ENLIGHT OMEGA assays are particularly suited to detect complex interactions prevailing between a series of bulky binding partners such as full-length proteins. In the example above, an ENLIGHT OMEGA assay was designed to detect anti-human IgGs specific to the SARS-CoV-2 protein (trimeric form) present in biological samples such as plasma, serum or saliva. Streptavidin-coated Sensibeads are used to capture biotinylated SARS-CoV-2 protein trimers used as baits. Anti SARS-CoV-2 IgGs present in a biological sample of interest bridge the Streptavidin-coated Sensibeads/biotinylated SARS-CoV-2 protein complex to the anti-human IgG coated Chemibeads. Both Sensibeads and Chemibeads brought into proximity then generate a signal proportional to the concentration of the anti SARS-CoV-2 IgGs content present in the biological sample.
Please refer to the following sections, should you want to have more details on ENLIGHT OMEGA:
BIOSIGNAL2 vs "Company R"
BIOSIGNAL2 vs "Company R"